不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响

doi:10.11843/j.issn.0366-6964.2015.01.012

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (1): 96-103.doi: 10.11843/j.issn.0366-6964.2015.01.012

不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响

郭鹏^1,2，刘大程^1,2*，赵培厅^1,2，高民³，胡红莲³

(1.内蒙古农业大学兽医学院，呼和浩特 010018;2.农业部动物疾病临床诊疗技术重点实验室，呼和浩特 010018;3.内蒙古农牧业科学院动物营养研究所，呼和浩特 010030)

收稿日期:2014-05-05 出版日期:2015-01-23 发布日期:2015-01-23
通讯作者: 刘大程，教授，主要从事反刍动物瘤胃微生态方面的研究，E-mail：nmgldc@163.com
作者简介:郭鹏(1991-)，女，满族，北京市人，硕士，主要从事反刍动物瘤胃微生态方面的研究，E-mail：bjhrgp@163.com
基金资助:
国家自然科学基金项目（30960252）；现代农业(奶牛)产业技术体系建设专项资金资助(CARS-37)

Effects of Increasing the Ratio of Non-fiber Carbohydrate to Neutral Detergent Fiber on Bacteria Flora，Endotoxin and Histamine Content in Rumen and Plasma of Dairy Goats

GUO Peng ^1，2，LIU Da-cheng^1，2*，ZHAO Pei-ting^1，2，GAO Min³，HU Hong-lian³

(1.College of Veterinary Medicine，Inner Mongolia Agricultural University，Hohhot 010018,China；2.Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease of Ministry of Agriculture，Hohhot 010018，China；3.Animal Nutrition Institute of Agriculture and Animal Husbandry，Academy of Inner Mongolia，Hohhot 010030，China)

Received:2014-05-05 Online:2015-01-23 Published:2015-01-23

摘要/Abstract

摘要：

旨在观察不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响。选用6只安装有永久性瘤胃瘘管的泌乳期关中奶山羊为试验动物，试验分四期进行，每期10 d，依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮，以逐渐增加日粮精料的方式诱导奶山羊发生亚急性瘤胃酸中毒（SARA）。分别利用pH动态监测记录系统和滚管培养法测定瘤胃pH、瘤胃细菌总数、淀粉分解菌及坏死梭形杆菌的浓度；以鲎试验试剂法和荧光分光光度法测定内毒素和组胺含量。结果表明：随着日粮NFC/NDF比的增大，瘤胃pH显著降低（P<0.05），并且瘤胃pH下降速率和下降幅度也随之加快；日粮NFC/NDF比为2.58时，瘤胃内组胺和内毒素的浓度明显增加（P<0.05），坏死梭形杆菌和淀粉分解菌的数量也显著升高（P<0.01）。内毒素、组胺和坏死梭形杆菌三者浓度的异常增加对SARA发生和发展起重要作用。

Abstract:

This experiment was conducted to study the effect of increasing the ratio of non-fiber carbohydrate to neutral detergent fiber on bacteria flora，endotoxin and histamine content in rumen and plasma of dairy goats.Six rumen-cannulated lactating Guanzhong goats were used to investigate the effects of increasing dietary NFC/NDF ratio (i.e.non-fiber carbohydrate to neutral detergent fiber) on bacteria flora，and endotoxin and histamine contents in the rumen and the plasma of dairy goats.Induction of subacute ruminal acidosis (SARA) was performed by increasing dietary NFC/NDF ratio from 1.02(stage Ⅰ) to 1.24(stage Ⅱ)，1.63(stage Ⅲ) and 2.58(stage Ⅳ).Each stage lasted 10 days.A dynamic pH monitoring system and a rolling-tube method were used to assess changes in ruminal pH and the numbers of total bacteria，amylolytic bacteria and Fusobacterium necrophorum in rumen.Limulus test and fluorescence spectrophotometry were used to assess the contents of endotoxin and histamine.Our results showed that as the dietary NFC/NDF ratio increased from 1.02 to 2.58，the rumen pH decreased significantly (P<0.05) at accelerated rates，while the endotoxin and histamine contents in the rumen and the numbers of amylolytic bacteria and necrotic fusiform bacilli increased significantly (P<0.01 and P<0.05 respectively).We conclude that abnormal increases of endotoxin，histamine and F.necrophorum played an important role in the occurrence and development of SARA.

中图分类号:

郭鹏，刘大程，赵培厅，高民，胡红莲. 不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响[J]. 畜牧兽医学报, 2015, 46(1): 96-103.

GUO Peng,LIU Da-cheng，ZHAO Pei-ting，GAO Min，HU Hong-lian. Effects of Increasing the Ratio of Non-fiber Carbohydrate to Neutral Detergent Fiber on Bacteria Flora，Endotoxin and Histamine Content in Rumen and Plasma of Dairy Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 96-103.

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不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响

Effects of Increasing the Ratio of Non-fiber Carbohydrate to Neutral Detergent Fiber on Bacteria Flora，Endotoxin and Histamine Content in Rumen and Plasma of Dairy Goats

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